BREEDING HABITAT PREFERENCES OF Aedes aegypti AS A DENGUE VECTOR IN URBAN AREAS OF BANJARMASIN, INDONESIA

Muhammad Rasyid Ridha, Misna Tazkiah, Ririh Yudhastuti, Yudi Yahya, Nurul Hidayah, Liestiana Indriati, Khairatun Nisa, Ahmad Budidarma, Isnawati Isnawati, Muhammad Pahruddin

Abstract


Dengue fever continues to be a public health challenge in Indonesia, especially in urban areas such as Banjarmasin City, where mosquito breeding is closely linked to household water storage practices. Despite ongoing ontrol efforts, limited entomological data on larval habitats hinders the implementation of targeted and effective vector control programs. This study aims to identify and describe the breeding habitat preferences of Aedes aegypti in urban areas, both endemic and non-endemic areas in Banjarmasin. A cross-sectional larval survey was conducted in 235 households across eight neighbourhoods, during which 948 water-holding containers were examined for mosquito larvae. Entomological indices, including the House Index (HI), Container Index (CI), and Breteau Index (BI), along with the Breeding Preference Ratio (BPR), were calculated. Associations between container characteristics and larval presence were assessed using chi-square tests and binary logistic regression analysis. Of all inspected containers, 21.7% were found positive for larvae. Although buckets were the most common, they were not the preferred breeding sites. Flower vases exhibited the highest BPR (5.20), indicating a greater likelihood of larval occurrence. Containers located outdoors were significantly associated with larval infestation (OR = 3.366; 95% CI: 1.522–7.444), while other factors such as container material, colour, presence of fish, and cleaning frequency showed no significant relationship. The results of this study highlight the influence of container type and placement on the breeding behaviour of Ae. aegypti. These findings support the implementation of behaviour-focused measures, namely prioritizing high-risk container types and encouraging routine cleaning and biological control to reduce the risk of dengue transmission.

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References


Agus, N.M., Srikandi, Y., Wijatmiko, Tri J., Nur Hidayah, Ririh, I., Octaviani, O., Gunawan, et al. 2023. Water containers and the preferable conditions for laying eggs by Aedes mosquitoes in Maros Regency, South of Sulawesi, Indonesia. Journal of Water and Health 21(11): 1741–46.

Aik, J., Neo, Z.W., Rajarethinam, J., Chio, K., Lam, W.M. & Ng, L-C. 2019. The effectiveness of inspections on reported mosquito larval habitats in households: A case-control study. PLoS Neglected Tropical Diseases 13(6): e0007492.

Barkhad, A., Lecours, N. & Mbuagbaw, L. 2025. Developing an eco-bio-social conceptual framework for dengue virus transmission in Latin America and the Caribbean: An e-Delphi study. PLOS Global Public Health 5(9): e0004115.

Barkhad, A., Lecours, N., Stevens-Uninsky, M. & Mbuagbaw, L. 2026. The relationships between the eco-bio-social determinants of dengue epidemiology in Latin America and the Caribbean: A scoping review of the literature. EcoHealth 30: 1–19.

Bowman, L.R., Runge-Ranzinger, S. & McCall, P.J. 2014. Assessing the relationship between vector indices and dengue transmission: A systematic review of the evidence. PLoS Neglected Tropical Diseases 8(5): e2848.

Chandrasegaran, K., Lahondère, C., Escobar, L.E. & Vinauger, C. 2020. Linking mosquito ecology, traits, behavior, and disease transmission. Trends in Parasitology 36(4): 393–403.

Combs, M.A., Kache, P.A., VanAcker, M.C., Gregory, N., Plimpton, L.D., Tufts, D.M., Fernandez, M.P. & Diuk-Wasser, M.A. 2022. Socio-ecological drivers of multiple zoonotic hazards in highly urbanized cities. Global Change Biology 28(5): 1705–24.

Cromwell, E.A., Stoddard, S.T., Barker, C.M., Van Rie, A., Messer, W.B., Meshnick, S.R., Morrison, A.C. & Scott, T.W. 2017. The relationship between entomological indicators of Aedes aegypti abundance and dengue virus infection. PLoS Neglected Tropical Diseases 11(3): e0005429.

Dharmamuthuraja, P.D.R., Lakshmi, M.I., Isvaran, K., Ghosh, S.K. & Ishtiaq, F. 2023. Determinants of Aedes mosquito larval ecology in a heterogeneous urban environment- a longitudinal study in Bengaluru, India. PLoS Neglected Tropical Diseases 17(11): e0011702.

Dagg, K.A., Estep, A.S., Bartz, C.E. & Burgess, E.R. 2025. Claustrophilic oviposition: Oviposition performance depends on container size in a novel forced oviposition method for Culex quinquefasciatus and Aedes aegypti. PLOS Neglected Tropical Diseases 19(7): e0013044.

Evans, K.G., Neale, Z.R., Holly, B., Canizela, C.C. & Juliano, S.A. 2022. Survival-larval density relationships in the field and their implications for control of container-dwelling Aedes mosquitoes. Insects 14(1): 17.

Fansiri, T., Buddhari, D., Pathawong, N., Pongsiri, A., Klungthong, C., Iamsirithaworn, S., Jones, A.R., Fernandez, S., Srikiatkhachorn, A., Rothman, A.L., Anderson, K.B., Thomas, S.J., Endy, T.P. & Ponlawat, A. 2021. Entomological risk assessment for dengue virus transmission during 2016-2020 in Kamphaeng Phet, Thailand. Pathogens 10(10): 1234.

Flaibani, N., Pérez, A.A., Barbero, I.M., & Burroni. N.E. 2020. Different approaches to characterize artificial breeding sites of Aedes Aegypti using generalized linear mixed models. Infectious Diseases of Poverty 9(04): 97–107.

Gibb, R., Colón-González, F.J., Lan, P.T., Huong, P.T., Nam, V.S., Duoc, V.T., Hung, D.T., Dong, N.T., Chien, V.C., Trang, L.T.T., Kien Quoc, D., Hoa, T.M., Tai, N.H., Hang, T.T., Tsarouchi, G., Ainscoe, E., Harpham, Q., Hofmann, B., Lumbroso, D., Brady, O.J. & Lowe, R. 2023. Interactions between climate change, urban infrastructure and mobility are driving dengue emergence in Vietnam. Nature Communications 14(1):8179.

Gouveia, A.S., Gomes, M.F.dc., de Almeida, I.F., Lana, R.M., Bastos, L.S., Bianchi, L.M., et al. 2025. Unraveling regional variability in dengue outbreaks in Brazil: Leveraging the Moving Epidemics Method (MEM) and climate data to optimize vector control strategies. PLOS Neglected Tropical Diseases 19(6): e0013175.

Gowelo, S., Meijer, P., Tizifa, T., Malenga, T., Mburu, M.M., Kabaghe, A.N., Terlouw, D.J., van Vugt, M., Phiri, K.S., Mzilahowa, T., Koenraadt, C.J.M., van den Berg, H., Manda-Taylor, L., McCann, R.S., Takken, W. 2022. Community participation in habitat management and larviciding for the control of malaria vectors in Southern Malawi. The American Journal of Tropical Medicine and Hygiene 108(1): 51-60.

Hashim, N.A. & Ahmad, A.H. 2025. Vector Breeding Site Information as a Tool for Transmission Threshold Analysis and Dengue Risk Assessment on Penang Island, Malaysia. Serangga 30(3): 87–107.

Herath, J.M.M.K., De Silva, W.A.P.P., Weeraratne, T.C. & Karunaratne, S.H.P.P. 2024. Breeding habitat preference of the dengue vector mosquitoes Aedes aegypti and Aedes albopictus from Urban, Semiurban, and Rural Areas in Kurunegala District, Sri Lanka. Journal of Tropical Medicine 2024(1): 4123543.

Hossain, M.J., Das, M., Islam, M.W., Shahjahan, M. & Ferdous, J. 2024. Community engagement and social participation in dengue prevention: A cross-sectional study in Dhaka City. Health Science Reports 7(4): e2022.

Indriyani, N., Ishak, H., Syamsuar, Ibrahim, E., Syahribulan & Masni. 2025. Water quality and density of Aedes Sp. larvae-a study from Indonesia. Indian Journal of Entomology 87(3): 579–582.

Jung, S.H., Kim, D., Jung, K-S. & Lee, D-K. 2021. Color preference for host-seeking activity of Aedes albopictus and Culex pipiens (Diptera: Culicidae). Journal of Medical Entomology 58(6): 2446–52.

Lakens, D. 2022. Sample size justification. Collabra: Psychology 8(1): 33267.

Little, E., Biehler, D., Leisnham, P.T., Jordan, R., Wilson, S. & LaDeau, S.L. 2017. Socio-ecological mechanisms supporting high densities of Aedes albopictus (Diptera: Culicidae) in Baltimore, MD. Journal of Medical Entomology 54(5): 1183-1192.

Macêdo, S.F., Silva, K.A., Vasconcelos, R.B., Sousa, I.V., Mesquita, L.P.S., Barakat, R.D.M., Fernandes, H.M.C., Queiroz, A.C.M., Santos, G.P.G., Filho, V.C.B., Carrasquilla, G., Caprara, A. & de Oliveira Lima, J.W. 2021. Scaling up of eco-bio-social strategy to control Aedes aegypti in highly vulnerable areas in Fortaleza, Brazil: A cluster, non-randomized controlled trial protocol. International Journal of Environmental Research and Public Health 18(3): 1278.

Mamenun, Koesmaryono, Y., Sopaheluwakan, A., Hidayati, R., Dasanto, B.D. & Aryati, R. 2024. Spatiotemporal characterization of dengue incidence and its correlation to climate parameters in Indonesia. Insects 15(5): 366.

Marston, Cicely, Renedo, A. & Miles, S. 2020. Community participation is crucial in a pandemic. The Lancet 395(10238): 1676–78.

Menda, G., Uhr, J.H., Wyttenbach, R.A., Vermeylen, F.M., Smith, D.M., Harrington, L.C. & Hoy, R.R. 2013. Associative learning in the dengue vector mosquito, Aedes aegypti: avoidance of a previously attractive odor or surface color that is paired with an aversive stimulus. Journal of Experimental Biology 216(2): 218–23.

Morales-Pérez, A., Nava-Aguilera, E., Hernández-Alvarez, C., Alvarado-Castro, V.M., Arosteguí, J., Legorreta-Soberanis, J., et al. 2020. Utility of entomological indices for predicting transmission of dengue virus: Secondary analysis of data from the Camino Verde trial in Mexico and Nicaragua. PLoS Neglected Tropical Diseases14(10): e0008768.

Nagaraj, P., Muneeswaran, V., Pandiaraj, A. & Jain, V. 2025. exploring healthcare data analytics for effective dengue prevention and control strategies. In. Chatterjee, J.M., Sujatha, R. & Saxena, S.K. (eds.). Role of Artificial Intelligence, Telehealth, and Telemedicine in Medical Virology, pp. 161–78. Singapore: Springer.

Norjanah, & Ridha, M.R. 2024. Spatial autocorrelation of dengue and its relationship with population density in South Kalimantan, Indonesia. Althea Medical Journal 11(2): 113–19.

Nurdin, Siregar, Y.I., Mubarak, M. & Wijayantono, W. 2022. Environmental factors linked to the presence of Aedes aegypti larvae and the prevalence of dengue hemorrhagic fever. Open Access Macedonian Journal of Medical Sciences 10(E): 475–80.

Nurjanah, S., Tri Atmowidi, Hadi, U.K., Solihin, D.D., Priawandiputra, W. & Meidaliyantisyah. 2023. Habitat preference of Aedes aegypti and Aedes albopictus: A case study on dengue endemic areas of Sumatera, Indonesia. Philippine Journal of Science 152(3): 1007–1014.

Oliva, L.O., Correia, J.C. & Albuquerque, C.M.R. 2014. How mosquito age and the type and color of oviposition sites modify skip-oviposition behavior in Aedes aegypti (Diptera: Culicidae)? Journal of Insect Behavior 27(1): 81–91.

Parker, A.T., McGill, K. & Allan, B.F. 2020. Container type affects mosquito (Diptera: Culicidae) oviposition choice. Journal of Medical Entomology 57(5): 1459–67.

Paz-Bailey, G., Adams, L.E., Deen, J., Anderson, K.B., Katzelnick, L.C. 2024. Dengue. Lancet 403(10427): 667-682.

Peterson, R.K.D. & Rolsto, M.G. 2022. Larval mosquito management and risk to aquatic ecosystems: A comparative approach including current tactics and gene-drive Anopheles techniques. Transgenic Research 31(4): 489–504.

Prasad, P., Lata, S., Gupta, S.K., Kumar, P., Saxena, R., Arya, D.K. & Singh, H. 2023. Aedes aegypti container preference for oviposition and its possible implications for dengue vector surveillance in Delhi, India. Epidemiology and Health 45: e2023073.

Ridha, M., Aisyah, S., Triana, Y., Priono, M. & Jumriadi, J. 2023. Improving community knowledge and behavior in the one house one Jumantik Program in dengue control. Jurnal Kesehatan Masyarakat 18(3): 423–30.

Ridha, M.R., Ferdina, A.R., Nita, R., Annida, Juhairiyah, Jumriadi & Misna, T. 2024. Supporting and inhibiting factors of implementation of dengue control in East Kalimantan: A qualitative study. Malaysian Journal of Medicine & Health Sciences 20: 186.

Ridha, M.R. & Sulasmi. 2022. Larval survey of the dengue-endemic area in Samarinda: Guide to determine risk containers. International Journal of Public Health Science (IJPHS) 11(4): 1176.

Ridha, M.R., Yudhastuti, R., Garjito, T.A., Hidajat, M.C., Juhairiyah, J., Indriati, L., Nita R., Diyanah, K.C., Jassey, B., Yahya, Y., Fajriannor, M., Nurul Hidayah, Nugraheni, P.D. & Anita, A.R. 2026. Incidence trend and climate influence on dengue fever in Banjarmasin, Indonesia: A path analysis approach. Journal of Health Science and Medical Research 44(1): 20251231.

Rueda, L.M. 2004. Pictorial keys for the identification of mosquitoes (Diptera: Culicidae) associated with Dengue Virus Transmission. Zootaxa 589(1): 1–60.

Ryan, S.J., Lippi, C.A., Nightingale, R., Hamerlinck, G., Borbor-Cordova, M.J., Manuel Cruz, B., Ortega, F., Leon, R., Waggoner, E. & Stewart-Ibarra, A.M. 2019. Socio-ecological factors associated with dengue risk and Aedes aegypti presence in the Galápagos Islands, Ecuador. International Journal of Environmental Research and Public Health 16(5): 682.

Saadatian-Elahi, M., Rabilloud, M., Möhlmann, T.W.R., Langlois-Jacques, C., Ariffin, F.D., Farenhorst, M., Elsensohn, M.H., Schmitt, F., Richardson, J.H., Baur, F., Leduc, M., Romli, N.N., Tan, L.K., Norazman, M.R., Shahar, H., Mudin, R.N., Alexander, N. & Ab Hamid, N. 2025. Effectiveness of integrated vector management on the incidence of dengue in urban Malaysia: A cluster-randomised controlled trial. Lancet Infectious Diseases 25(9): 977–85.

Sauer, F.G., Grave, J., Lühken, R. & Kiel, E. 2021. Habitat and microclimate affect the resting site selection of mosquitoes. Medical and Veterinary Entomology 35(3): 379–88.

Shafique, M., Lopes, S., Doum, D., Keo, V., Sokha, L., Sam, B., Vibol, C., Alexander, N., Bradley, J., Liverani, M., Hii, J., Rithea, L., Aryal, S. & Hustedt, J. 2019. Implementation of guppy fish (Poecilia reticulata), and a novel larvicide (Pyriproxyfen) product (Sumilarv 2MR) for dengue control in Cambodia: A qualitative study of acceptability, sustainability and community engagement. PLoS Neglected Tropical Diseases 13 (11): e0007907.

Soto-López, J.D., Barrios-Izás, M.A., Vieira L.M.C. & Muro, A. 2024. Role of non-residential larval habitats in Aedes spatiotemporal egg production. Life 14(8): 1013.

Sukiato, F., Wasserman, R.J., Foo, S.C., Wilson, R.F. & Cuthbert, R.N. 2019. The effects of temperature and shading on mortality and development rates of Aedes aegypti (Diptera: Culicidae). Journal of Vector Ecology 44(2): 264–70.

Wanti, W., Yudhastuti, R., Yotopranoto, S., Notobroto, H.B. Sri Subekti & Umniati, S.R. 2017. Container positivity and larva distribution based on the container characteristics. International Journal of Public Health Science 6(3): 237–42.

WHO. 2009. Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control. French: World Health Organization.

WHO. 2016. Technical Handbook for Dengue Surveillance, Outbreak Prediction/Detection and Outbreak Response. Geneva, Switzerland: World Health Organization.

Wilson, A.L., Courtenay, O., Kelly-Hope, L.A., Scott, T.W., Takken, W., Torr, S.J., Lindsay, S.W. 2020. The importance of vector control for the control and elimination of vector-borne diseases. PLoS Neglected Tropical Diseases 14 (1): e0007831.

Yin, S., Ren, C., Shi, Y., Hua, J., Yuan, H-Y. & Tian, L-W. 2022. A systematic review on modeling methods and influential factors for mapping dengue-related risk in urban settings. International Journal of Environmental Research and Public Health 19(22): 15265.


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