EVALUATING THE EFFICACY OF COMMERCIAL Bacillus thuringiensis israelensis FOR Aedes albopictus LARVAL CONTROL IN VARIOUS LAND USE
Abstract
A commercial bacterial product of Bacillus thuringiensis israelensis (Bti); Mosquito BTI from USA was tested for its efficacy in exterminating the local populations of Aedes albopictus larvae. Larval surveys were carried out to obtain field populations of Ae. albopictus from various land use namely dengue-risk and dengue-free housing localities as well as agrarian localities like oil palm plantations, rubber estates and paddy fields. A 24-hour larval bioassay using Mosquito BTI was performed on each Ae. albopictus population. Only a maximum of 2.00% mortality was observed by the end of the first four hours of exposure while a maximum of 25.00% mortality was displayed at 24 hours of exposure. Results showed the potential use of Bti in reducing local Ae. albopictus larval populations but more than 24 hours may be required to achieve complete mortalities among these populations upon exposure to Bti. Some limitations of this study were also discussed.
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Alam, I., Batool, K., Idris, A.L., Tan, W., Guan, X. & Zhang, L. 2022. Function of CTLGA9 amino acid residue leucine-6 in modulating cry toxicity. Frontiers in Immunology 13: 906259.
Asgarian, T.S., Vatandoost, H., Hanafi-Bojd, A.A. & Nikpoor, F. 2023. Worldwide status of insecticide resistance of Aedes aegypti and Aedes albopictus vectors of arboviruses of chikungunya, dengue, Zika and yellow fever. Journal of Arthropod-Borne Diseases 17(1): 1-27.
Balaska, S., Fotakis, E.A., Kioulos, I., Grigoraki, L., Mpellou, S., Chaskopoulou, A. & Vontas, J. 2020. Bioassay and molecular monitoring of insecticide resistance status in Aedes albopictus populations from Greece, to support evidence-based vector control. Parasites & Vectors 13(1): 328.
Bohari, R., Hin, C.J., Matusop, A., Abdullah, M.R., Ney, T.G., Benjamin, S. & Lim, L.H. 2020. Wide area spray of bacterial larvicide, Bacillus thuringiensis israelensis strain AM65-52, integrated in the national vector control program impacts dengue transmission in an urban township in Sibu district, Sarawak, Malaysia. PLoS ONE 15(4): e0230910.
Carvalho, K.d.S., Guedes, D.R.D., Crespo, M.M., Melo-Santos, M.A.V. & Silva-Filha, M.H.N.L. 2021. Aedes aegypti continuously exposed to Bacillus thuringiensis svar. israelensis does not exhibit changes in life traits but displays increased susceptibility for Zika virus. Parasites & Vectors 14: 379.
Department of Agriculture Peninsular Malaysia. 2015. Paddy statistics of Malaysia 2014. Malaysia: Department of Agriculture, Peninsular Malaysia, Malaysia.
Derua, Y.A., Tungu, P.K., Malima, R.C., Mwingira, V., Kimambo, A.G., Batengana, B.M., Machafuko, P., Sambu, E.Z., Mgaya, Y.D. & Kisinza, W.N. 2022. Laboratory and semi-field evaluation of the efficacy of Bacillus thuringiensis var. israelensis (Bactivec®) and Bacillus sphaericus (Griselesf®) for control of mosquito vectors in northeastern Tanzania. Current Research in Parasitology & Vector-Borne Diseases 2: 100089.
Elia-Amira, N.M.R., Chen, C.D., Lau, K.W., Lee, H.L., Low, V.L., Norma-Rashid, Y. & Sofian-Azirun, M. 2018. Organophosphate and organochlorine resistance in larval stage of Aedes albopictus (Diptera: Culicidae) in Sabah, Malaysia. Journal of Economic Entomology 111(5): 2488-2492.
Ferreira, Q.R., Lemos, F.F.B., Moura, M.N., Nascimento, J.O.d.S., Novaes, A.F., Barcelos, I.S., Fernandes, L.A., Amaral, L.S.d.B., Barreto, F.K. & Melo, F.F.d. 2023. Role of the microbiome in Aedes spp. vector competence: What do we know? Viruses 15(3): 779.
Jeffery, J., Rohela, M., Muslimin, M., Abdul Aziz, S.M.N., Jamaiah, I., Kumar, S.Tan, T.C., Lim, Y.A.L., Nissapatorn, V. & Abdul-Aziz, N.M. 2012. Illustrated Keys: Some Mosquitoes of Peninsula Malaysia. Kuala Lumpur, Malaysia: University of Malaya Press.
Odom, O.W., Kang, S., Ferguson, C., Chen, C. & Herrin, D.L. 2022. Overcoming poor transgene expression in the wild-type Chlamydomonas chloroplast: Creation of highly mosquitocidal strains of Chlamydomonas reinhardtii. Microorganisms 10(6): 1087.
Rajendran, D., Adnan, F.N., Besar, A.U.A., Yusoff, M. & Zuharah, W.F. 2021. Status of insecticide resistance on Aedes aegypti (L.) and Aedes albopictus (Skuse) in Kampar, Perak, Malaysia. Serangga 26(2): 245-254.
Rasli, R., Cheong, Y.L., Ibrahim, M.K.C., Fikri, S.F.F., Norzali, R. N., Nazarudin, NA., Hamdan, N. F., Muhamed, K.A., Hafisool, A.A., Azmi, R.A., Ismail, H.A., Ali, R., Hamid, N.A., Taib, M.Z., Omar, T., Ahmad, N.W. & Lee, H.L. 2021. Insecticide resistance in dengue vectors from hotspots in Selangor, Malaysia. PLoS Neglected Tropical Diseases 15(3): e0009205.
Sakinah, S., Priya, S.P., Mok, P.L., Munisvaradass, R., Teh, S.W., Sun, Z., Alzahrani, B., Abu Bakar, F., Chee, H.-Y., Awang Hamat, R., He, G., Xiong, C., Joseph, N., Tong, J.B., Wu, X., Maniam, M., Samrot, A.V., Higuchi, A. & Kumar, S.S. 2021. Stem cell therapy in dengue virus-infected BALB/C mice improves hepatic injury. Frontiers in Cell and Developmental Biology 9: 637270.
Su, X., Guo, Y., Deng, J., Xu, J., Zhou, G., Zhou, T., Li, Y., Zhong, D., Kong, L., Wang, X., Liu, M., Wu, K., Yan, G. & Chen, X. G. 2019. Fast emerging insecticide resistance in Aedes albopictus in Guangzhou, China: Alarm to the dengue epidemic. PLoS Neglected Tropical Diseases 13(9): e0007665.
Suter, T., Crespo, M.M., Oliveira, M.F., Oliveira, T.S.A., Melo-Santos, M.A.V., Olioveira, C.M.F., Ayres, C.F.J., Barbosa, RM. R., Araujo, A.P., Regis, L.N., Flacio, E., Engeler, L., Muller, P. & Silva-Filha, MH. N. 2017. Insecticide susceptibility of Aedes albopictus and Ae. aegypti from Brazil and the Swiss-Italian border region. Parasites & Vectors 10(1): 431.
Wan-Norafikah, O., Nazni, W.A., Lee, H.L., Zainol-Ariffin, P. & Sofian-Azirun, M. 2010. Permethrin resistance in Aedes aegypti (Linnaeus) collected from Kuala Lumpur, Malaysia. Journal of Asia-Pacific Entomology 13(3): 175-182.
World Health Organization 2016. Monitoring and Managing Insecticide Resistance in Aedes Mosquito Populations. Interim Guidance for Entomologists (WHO/ZIKV/VC/16.1). Geneva, Switzerland: World Health Organization.
Yougang, A.P., Kamgang, B., Tedjou, A.N., Wilson-Bahun, T.A., Njiokou, F. & Wondji, C.S. 2020. Nationwide profiling of insecticide resistance in Aedes albopictus (Diptera: Culicidae) in Cameroon. PLoS ONE 15(6): e0234572.
Zaidi, N.J., Abdullah, A.A., Heh, C.H., Lin, C.-H., Othman, R. & Ahmad Fuaad, A.A.H. 2022. Hit-to-lead short peptides against dengue type 2 envelope protein: Computational and experimental investigations. Molecules 27(10): 3233.
Zaki, Z.A., Dom, N.C. & Alhothily, I.A. 2020. Efficacy of Bacillus thuringiensis treatment on Aedes population using different applications at high-rise buildings. Tropical Medicine and Infectious Disease 5(2): 67.
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